nucB [2017-11-28 10:31:16]

sporulation-specific extracellular nuclease

locus: BSU25750

pI: 5.17

mw: 14.83 kDa

protein length: 136 aa

gene length: 408 bp

function: DNA degradation after mother cell lysis

product: sporulation-specific extracellular nuclease

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 2,652,387 → 2,652,797

The protein

[SW|Localization]

• extracellular(signal peptide)(according to UniProt)

Expression and Regulation

Operons

• genes: [gene|5A3CEDBF81E3263FDB10AC9245D28498C9332D14|nucB]
  description: [Pubmed|7746143]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|7746143], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • expressed during sporulation ([protein|search|SigE]) [Pubmed|7746143]

Biological materials

Mutant

• BKE25750 (Δ[gene|5A3CEDBF81E3263FDB10AC9245D28498C9332D14|nucB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCCATCCCCCCATACC, downstream forward: _UP4_TAGTAAAGAAGAGGCTCTTT
• BKK25750 (Δ[gene|5A3CEDBF81E3263FDB10AC9245D28498C9332D14|nucB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCCATCCCCCCATACC, downstream forward: _UP4_TAGTAAAGAAGAGGCTCTTT

References

• 17209033,7746143